qPCR Efficiency Calculator
Calculate PCR amplification efficiency from a standard-curve slope.
Enter a negative slope (e.g. -3.32).
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How to use this calculator
Enter the slope of your log-quantity vs Ct standard curve. A slope of −3.32 corresponds to 100% efficiency.
Formula / method used
Efficiency = (10^(−1/slope) − 1) × 100%